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Kosmos
Astronomia Astrofizyka
Inne

Kultura
Sztuka dawna i współczesna, muzea i kolekcje

Metoda
Metodologia nauk, Matematyka, Filozofia, Miary i wagi, Pomiary

Materia
Substancje, reakcje, energia
Fizyka, chemia i inżynieria materiałowa

Człowiek
Antropologia kulturowa Socjologia Psychologia Zdrowie i medycyna

Wizje
Przewidywania Kosmologia Religie Ideologia Polityka

Ziemia
Geologia, geofizyka, geochemia, środowisko przyrodnicze

Życie
Biologia, biologia molekularna i genetyka

Cyberprzestrzeń
Technologia cyberprzestrzeni, cyberkultura, media i komunikacja

Działalność
Wiadomości | Gospodarka, biznes, zarządzanie, ekonomia

Technologie
Budownictwo, energetyka, transport, wytwarzanie, technologie informacyjne

ESCHERICHIA COLI PATHOTYPES ASSOCIATED WITH DIARRHEA IN HUMAN AND DOMESTIC ANIMALS

Ruminants are important reservoirs for zoonotic pathogenic E. coli. The objective of this study was to characterize pathogenic E. coli isolates from cattle and sheep linked to human illness with respect to their pathotypes, serotypes and genotypes. E. coli O157:H7 isolated from cattle, sheep and human patients were compared for their genomic similarity by Pulsed-Field Gel-Electrophoresis (PFGE). PCR detection of virulence factors associated with different E. coli pathotypes (VTEC, ETEC, EPEC, EAEC and EIEC) revealed that VTEC was the most prevalent pathotype (22/45; 48.9%), followed by EAEC (3/45; 6.7%), EPEC (1/45; 2.2%), a EPEC (3/45; 6.7%), ETEC (1/45; 2.2%) and EIEC (1/45; 2.2%). E. coli O157:H7 represented the most prevalent VTEC serotypes (11/22; 50%). Pulsed field gel electrophoresis typing revealed exact matches between E. coli O157:H7 isolates from the human patients, sheep and cattle in the same municipality. VTEC play an important cause of diarrhea in human, sheep and cattle. The molecular relatedness between PFGE profiles of E. coli O157:H7 isolates from human, sheep and cattle supported the hypothesis that ruminants especially cattle and sheep act as reservoirs of E. coli O157:H7 for human infection.